{"id":338577,"date":"2024-10-19T23:41:40","date_gmt":"2024-10-19T23:41:40","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-172802019\/"},"modified":"2024-10-25T22:47:51","modified_gmt":"2024-10-25T22:47:51","slug":"bs-en-172802019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-172802019\/","title":{"rendered":"BS EN 17280:2019"},"content":{"rendered":"

This document specifies a procedure for the determination of nivalenol (NIV), deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON), HT-2 and T-2 toxins (HT-2 and T-2) and zearalenone (ZEN) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS\/MS) after clean-up by solid phase extraction (SPE).<\/p>\n

The method has been validated with samples of wheat, wheat flour, and wheat crackers. The wheat and the wheat flour were prepared from a mixture of wheat and fungi infected wheat kernels. The wheat crackers were baked from wheat flour and water spiked with the target mycotoxins.<\/p>\n

Validation levels for NIV ranged from 27,7 ?g\/kg to 378 ?g\/kg.<\/p>\n

Validation levels for DON ranged from 234 ?g\/kg to 2420 ?g\/kg.<\/p>\n

Validation levels for 3-acetyl-DON ranged from 18,5 ?g\/kg to 137 ?g\/kg.<\/p>\n

Validation levels for 15-acetyl-DON ranged from 11,4 ?g\/kg to 142 ?g\/kg.<\/p>\n

Validation levels for HT-2 ranged from 6,6 ?g\/kg to 134 ?g\/kg.<\/p>\n

Validation levels for T-2 ranged from 2,1 ?g\/kg to 37,6 ?g\/kg.<\/p>\n

Validation levels for ZEN ranged from 31,6 ?g\/kg to 230 ?g\/kg.<\/p>\n

Laboratory experiences have shown that this method is also applicable to barley and oat flour, and rye based crackers [5<\/span>], however, this has not been validated in a collaborative study.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Principle <\/td>\n<\/tr>\n
8<\/td>\n5 Reagents <\/td>\n<\/tr>\n
10<\/td>\n6 Apparatus and equipment <\/td>\n<\/tr>\n
11<\/td>\n7 Procedure
7.1 Preparation of the test sample
7.2 Extraction
7.3 Solid phase extraction clean up
7.4 Preparation of the sample test solution
7.5 Spiking procedure <\/td>\n<\/tr>\n
12<\/td>\n7.6 LC-MS\/MS analysis
7.6.1 General
7.6.2 Injection sequence
7.7 Identification
7.8 Calibration <\/td>\n<\/tr>\n
13<\/td>\n8 Calculation
9 Precision
9.1 General
9.2 Repeatability
9.3 Reproducibility <\/td>\n<\/tr>\n
17<\/td>\n10 Test report <\/td>\n<\/tr>\n
18<\/td>\nAnnex A (informative)Typical chromatograms
A.1 Example chromatogram using the settings according to B.1 <\/td>\n<\/tr>\n
19<\/td>\nA.2 Example chromatogram using the settings according to B.2 <\/td>\n<\/tr>\n
20<\/td>\nAnnex B (informative)Example conditions for suitable LC-MS\/MS systems
B.1 System Settings for API SCIEX 5000\u2122,
B.1.1 Settings for chromatography <\/td>\n<\/tr>\n
21<\/td>\nB.1.2 Detector parameters <\/td>\n<\/tr>\n
22<\/td>\nB.2 System settings for Thermo Q-Exactive\u2122,
B.2.1 Settings for chromatography <\/td>\n<\/tr>\n
23<\/td>\nB.2.2 Detector Parameters <\/td>\n<\/tr>\n
26<\/td>\nAnnex C (informative)Additional examples for separation between 3-AcDON and 15-AcDON
C.1 Settings for AccucoreTM C18 (Thermo Scientific) <\/td>\n<\/tr>\n
27<\/td>\nC.2 Settings for Kinetex\u00ae C18 (Phenomenex)4
C.3 Settings for Kinetex\u00ae PFP (Phenomenex) <\/td>\n<\/tr>\n
29<\/td>\nAnnex D (informative)Precision data <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Determination of zearalenone and trichothecenes including deoxynivalenol and its acetylated derivatives (3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol), nivalenol T-2 toxin and HT-2 toxin in cereals and cereal products by LC-MS\/MS<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2019<\/td>\n52<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338584,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338577","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338577","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338584"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338577"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338577"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338577"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}