This European Standard specifies a method for the determination of benzene in soft drinks, other beverages and vegetable-based infant foods, by headspace gas chromatography mass spectrometry (HS-GC-MS). The method has been validated in an interlaboratory study via the analysis of spiked samples of carbonated soft drink, still fruit-based drink, carbonated fruit-based drink, vegetable and fruit juice containing carrot, infant food vegetable based and infant food containing meat, ranging from 1,9 \u00b5g\/kg to 18,6 \u00b5g\/kg. However, linearity of the instrument response was proven for the concentration range from 0,5 \u00b5g\/kg to 20 \u00b5g\/kg. The limit of quantification (LOQ) depends on the instrument but can generally be expected to be in the range from 0,5 \u00b5g\/kg to 1,0 \u00b5g\/kg.<\/p>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 2<\/td>\n | National foreword <\/td>\n<\/tr>\n | ||||||
| 6<\/td>\n | 1 Scope 2 Normative references 3 Principle 4 Reagents 4.1 Benzene, C6H6, purity is\u00a0\u2265\u00a099,0\u00a0% (CAS\u00a071-43-2). 4.2 Benzene-d6, purity is\u00a0\u2265\u00a099, 6 atom %\u00a0D (CAS\u00a01076-43-3). 4.3 Helium purified compressed gas, purity equivalent to 99,995\u00a0% or better. 4.4 Methanol, suitable for headspace GC analysis (CAS 67-56-1). 4.5 Preparation of stock and standard solutions 4.5.1 General <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | 4.5.2 Benzene stock solution, mass concentration \u03c1 approximately 2,20\u00a0mg\/ml 4.5.3 Benzene intermediate standard solution, \u03c1 approximately 54\u00a0\u00b5g\/ml 4.5.4 Benzene standard solution, \u03c1\u00a0approximately 0,5\u00a0\u00b5g\/ml 4.5.5 Benzene-d6 internal standard (IS) stock solution, \u03c1 approximately 2,36\u00a0mg\/ml 4.5.6 Benzene-d6 internal standard (IS) solution, \u03c1\u00a0approximately 4,2\u00a0\u00b5g\/ml 4.6 Preparation of the calibration solutions <\/td>\n<\/tr>\n | ||||||
| 8<\/td>\n | 5 Apparatus <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | 6 Procedure 6.1 General 6.2 Sample treatment 6.3 Test sample preparation 7 HS-GC-MS analysis 7.1 General <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 7.2 Headspace sampling conditions 7.3 Injection conditions for split\/splitless injector 7.4 Oven conditions 7.5 Column conditions 7.6 Transfer line conditions 7.7 Mass spectrometer conditions 7.8 Mass spectrometer acquisition parameters and peak identification <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 7.9 Calibration curve 7.10 Sample analysis <\/td>\n<\/tr>\n | ||||||
| 13<\/td>\n | 8 Calculation 9 Precision data 9.1 General 9.2 Repeatability <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 9.3 Reproducibility 10 Test report <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | Annex\u00a0A (informative)Typical chromatograms <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | Annex\u00a0B (informative)Precision data <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Foodstuffs. Determination of benzene in soft drinks, other beverages and vegetable-based infant foods by headspace gas chromatography mass spectrometry (HS-GC-MS)<\/b><\/p>\n |