ISO 23418:2022

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Microbiology of the food chain — Whole genome sequencing for typing and genomic characterization of bacteria — General requirements and guidance

Published By	Publication Date	Number of Pages
ISO	2022-06	52

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Categories: 07.100.30 - Food microbiology, ISO

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Description

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:

a) handling of bacterial cultures;

b) axenic genomic DNA isolation;

c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;

d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;

e) metadata capture and sequence repository deposition;

f) validation of the end-to-end WGS workflow (fit for purpose for intended application).

This document is applicable to bacteria isolated from:

— products intended for human consumption;

— products intended for animal feed;

— environmental samples from food and feed handling and production areas;

— samples from the primary production stage.

Additional information

Published Code	ISO
Published By	International Organization for Standardization
Publication Date	2022-06
Pages Count	52
Language	France
Edition	1
File Size	1.5 MB
ICS Codes	07.100.30 - Food microbiology

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ISO 23418:2022

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