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BS ISO 19290:2021

BS ISO 19290:2021

$102.76

Cigarettes. Determination of tobacco specific nitrosamines in mainstream cigarette smoke.Method using LC-MS/MS

Published By Publication Date Number of Pages
BSI 2021 22
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This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of mainstream cigarette smoke by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Principle
5 Apparatus
6 Reagents
10 7 Preparation
7.1 Preparation of glassware
7.2 Preparation of solutions
11 7.3 Preparation of standards
7.3.1 General
7.3.2 Preparation of internal standard solutions
7.3.3 Preparation of calibration standard solutions
13 8 Sampling
9 Tobacco product preparation
10 Sample generation — Smoking of cigarettes
10.1 General
10.2 Linear smoking
10.3 Rotary smoking
11 Sample analysis
11.1 Preparation of sample
11.1.1 General
11.1.2 Extraction for linear smoking (44 mm pad)
11.1.3 Extraction for rotary smoking (92 mm pad)
11.1.4 Final sample preparation
14 11.2 Reversed phase high performance liquid chromatography
11.2.1 General
11.2.2 HPLC set-up parameters (Example)
11.2.3 Mobile phase (Example)
11.2.4 Mobile phase: Gradient (Example)
11.2.5 MS/MS Set-up parameters (Example)
15 11.3 Calculation
11.3.1 Calibration curve
16 11.3.2 Determination of the TSNAs concentrations
11.3.3 Sample quantification
11.3.4 Determination of mainstream smoke TSNA deliveries
12 Repeatability and reproducibility
18 13 Test report
19 Annex A (informative) Example of typical chromatograms
21 Bibliography

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BS ISO 19290:2021
$102.76