BS EN ISO 22119:2011

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Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions

Published By	Publication Date	Number of Pages
BSI	2011	24

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Categories: 07.100.30 - Food microbiology, BSI

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Description

This International Standard defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). This International Standard also specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.

The minimum requirements laid down in this International Standard provide the basis for comparable and reproducible results within individual and between different laboratories.

This International Standard is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

NOTE Because of the rapid progress in this field, the examples given are those most frequently in use at the time of development of this International Standard.

PDF Catalog

PDF Pages	PDF Title
9	Scope Normative references Terms and definitions
13	Principle General Probes for real-time PCR Hydrolysis probes Hybridization probes
14	Molecular beacons General laboratory requirements Reagents and materials
15	Apparatus
16	Laboratory sample Procedure Sample preparation Amplification General Cycling parameters Hydrolysis probes Hybridization probes
17	Molecular beacons Controls Analysis of the fluorescence data Amplification plot General
18	Phase 1: Exponential phase Phase 2: Linear phase Phase 3: Plateau phase Evaluation of the fluorescence data Quantitative analysis General Standard curve method for quantification
19	Evaluation and documentation

Additional information

Status	Under Review
Pages	24
Publication Date	2011-08-31
ISBN	978 0 580 60377 8
Standard Number	BS EN ISO 22119:2011
Title	Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions
Identical National Standard Of	EN ISO 22119:2011, ISO 22119:2011
Descriptors	Enzymes, Biochemical methods of analysis, Terminology, Definitions, Microbiology, Food testing, Ribonucleic acid, Pathogens, Microbiological analysis, Deoxyribonucleic acid, Food products, Animal feed, Nucleic acids, Microorganisms
Publisher	BSI
Committee	AW/9
ICS Codes	07.100.30 - Food microbiology

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BS EN ISO 22119:2011

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