BS EN ISO 16014-5:2019
$142.49
Plastics. Determination of average molecular weight and molecular weight distribution of polymers using size-exclusion chromatography – Light-scattering method
| Published By | Publication Date | Number of Pages |
| BSI | 2019 | 34 |
This document specifies a general method for determining the average molecular weight and the molecular weight distribution of polymers using SEC-LS, i.e. size-exclusion chromatography coupled with light-scattering detection. The average molecular weight and the molecular weight distribution are calculated from molecular weight data and weight concentrations determined continuously with elution time. The molecular weight at each elution time is determined absolutely by combining a light-scattering detector with a concentration-sensitive detector. Therefore, SEC-LS is classified as an absolute method.
This method is applicable to linear homopolymers and to nonlinear homopolymers such as branched, star-shaped, comb-like, stereo-regular and stereo-irregular polymers. It can also be applied to heterophasic copolymers whose molecular composition cannot vary. However, SEC-LS is not applicable to block, graft or heterophasic copolymers whose molecular composition can vary. And the methods are applicable to molecular weights ranging from that of the monomer to 3 000 000, but are not intended for samples that contain > 30 % of components having a molecular weight < 1 000.
This document specifies a general method for determining the average molecular weight and the molecular weight distribution of polymers using SEC-LS, i.e. size-exclusion chromatography coupled with light-scattering detection. The average molecular weight and the molecular weight distribution are calculated from molecular weight data and weight concentrations determined continuously with elution time. The molecular weight at each elution time is determined absolutely by combining a light-scattering detector with a concentration-sensitive detector. Therefore, SEC-LS is classified as an absolute method.
This method is applicable to linear homopolymers and to nonlinear homopolymers such as branched, star-shaped, comb-like, stereo-regular and stereo-irregular polymers. It can also be applied to heterophasic copolymers whose molecular composition cannot vary. However, SEC-LS is not applicable to block, graft or heterophasic copolymers whose molecular composition can vary. And the methods are applicable to molecular weights ranging from that of the monomer to 3 000 000, but are not intended for samples that contain > 30 % of components having a molecular weight < 1 000.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 4 | European foreword |
| 7 | Foreword |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Symbols 5 Principle 5.1 SEC 5.2 Light-scattering SEC |
| 11 | 6 Reagents 6.1 Eluent. 6.2 Reagent for column evaluation 6.3 Calibration standards 7 Apparatus 7.1 General |
| 12 | 7.5.1 General |
| 13 | 8 Procedure 8.1 Preparation of calibration solutions 8.2 Preparation of a solution for determining the L-point 8.3 Preparation of sample solutions 8.4 Preparation of solutions for column performance evaluation 8.5 Setting up the apparatus 8.6 Operating parameters 8.6.1 Flow rate 8.6.2 Injection masses and injection volumes |
| 14 | 8.6.3 Column temperature 8.6.4 Detector sensitivity 8.7 Number of determinations 9 Calibration 9.1 Calibration of concentration-sensitive detector and light-scattering detector 9.1.1 General 9.1.2 Calibration method A |
| 15 | 9.1.3 Calibration method B 9.1.4 Calibration method C |
| 16 | 9.2 Determination of delay volume 9.3 Normalization of detector sensitivity 9.4 Determination of refractive index increment 10 Data acquisition and processing 10.1 Data acquisition 10.2 Evaluation of data and correction of chromatograms 10.3 Data processing 10.3.1 Baseline determination |
| 17 | 10.3.2 Determination of calculation range 10.3.3 Calculation of signal intensity 10.3.4 Calculation of molecular weight 10.3.5 Second virial coefficient, A2 11 Expression of results 11.1 Calibration curve 11.1.1 General |
| 18 | 11.1.2 Method A 11.1.3 Method B |
| 20 | 11.2 Calculation of average molecular weight 11.3 Differential molecular weight distribution curve 11.4 Cumulative molecular weight distribution curve |
| 21 | 12 Precision 13 Test report 13.1 General 13.2 Apparatus and measurement parameters 13.3 Calibration of the system |
| 22 | 13.4 Calibration curve 13.5 Results |
| 23 | Annex A (informative) Interlaboratory test |
| 25 | Annex B (informative) Information on light scattering |
| 29 | Annex C (informative) Calibration curve in low molecular weight range |
| 31 | Bibliography |
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ISO 16014-5:2019
Plastics — Determination of average molecular weight and molecular weight distribution of polymers using size-exclusion…
