This document specifies a method, by measuring the oxygen demand in a closed respirometer, for the determination of the degree of aerobic biodegradability of plastic materials, including those containing formulation additives. The test material is exposed in an aqueous medium under laboratory conditions to an inoculum from activated sludge.<\/p>\n
If an unadapted activated sludge is used as the inoculum, the test simulates the biodegradation processes which occur in a natural aqueous environment; if a mixed or pre-exposed inoculum is used, the method is used to investigate the potential biodegradability of a test material.<\/p>\n
The conditions used in this document do not necessarily correspond to the optimum conditions allowing maximum biodegradation to occur, but this document is designed to determine the potential biodegradability of plastic materials or give an indication of their biodegradability in natural environments.<\/p>\n
The method enables the assessment of the biodegradability to be improved by calculating a carbon balance (optional, see Annex E).<\/p>\n
The method applies to the following materials.<\/p>\n
Natural and\/or synthetic polymers, copolymers or mixtures thereof.<\/p>\n<\/li>\n
Plastic materials which contain additives such as plasticizers, colorants or other compounds.<\/p>\n<\/li>\n
Water-soluble polymers.<\/p>\n<\/li>\n
Materials which, under the test conditions, do not inhibit the microorganisms present in the inoculum. Inhibitory effects can be determined using an inhibition control or by another appropriate method (see, for example, ISO 8192[<\/sup>2<\/sup>]<\/sup>). If the test material is inhibitory to the inoculum, a lower test concentration, another inoculum or a pre-exposed inoculum can be used.<\/p>\n<\/li>\n<\/ul>\n Determination of the ultimate aerobic biodegradability of plastic materials in an aqueous medium. Method by measuring the oxygen demand in a closed respirometer<\/b><\/p>\nPDF Catalog<\/h4>\n
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\n PDF Pages<\/th>\n PDF Title<\/th>\n<\/tr>\n \n 2<\/td>\n National foreword <\/td>\n<\/tr>\n \n 4<\/td>\n European foreword <\/td>\n<\/tr>\n \n 6<\/td>\n Foreword <\/td>\n<\/tr>\n \n 8<\/td>\n Introduction <\/td>\n<\/tr>\n \n 9<\/td>\n 1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n\n 11<\/td>\n 4 Principle
5 Test environment
6 Reagents <\/td>\n<\/tr>\n\n 12<\/td>\n 6.1 Distilled or deionized water
6.2 Test medium
6.2.1 Standard test medium
6.2.2 Optimized test medium <\/td>\n<\/tr>\n\n 14<\/td>\n 6.3 Pyrophosphate solution
6.4 Carbon dioxide absorber
7 Apparatus
8 Procedure
8.1 Test material <\/td>\n<\/tr>\n\n 15<\/td>\n 8.2 Reference material
8.3 Preparation of the inoculum <\/td>\n<\/tr>\n\n 16<\/td>\n 8.4 Test <\/td>\n<\/tr>\n \n 17<\/td>\n 9 Calculation and expression of results
9.1 Calculation <\/td>\n<\/tr>\n\n 18<\/td>\n 9.2 Expression and interpretation of results
10 Validity of results <\/td>\n<\/tr>\n\n 19<\/td>\n 11 Test report <\/td>\n<\/tr>\n \n 20<\/td>\n Annex A (informative) Theoretical oxygen demand (ThOD) <\/td>\n<\/tr>\n \n 21<\/td>\n Annex B (informative) Correction of BOD values for interference by nitrification <\/td>\n<\/tr>\n \n 23<\/td>\n Annex C (informative) Principle of a closed manometric respirometer <\/td>\n<\/tr>\n \n 25<\/td>\n Annex D (informative) Two-phase closed-bottle version of the respirometric test <\/td>\n<\/tr>\n \n 28<\/td>\n Annex E (informative) Example of the determination of a carbon balance <\/td>\n<\/tr>\n \n 30<\/td>\n Annex F (informative) Example of a determination of the amount of water-insoluble polymer remaining at the end of a biodegradation test and the molecular mass of the polymer <\/td>\n<\/tr>\n \n 31<\/td>\n Annex G (informative) Example of the determination of the CO2 absorbed in the absorbent <\/td>\n<\/tr>\n \n 33<\/td>\n Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" \n\n
\n Published By<\/td>\n Publication Date<\/td>\n Number of Pages<\/td>\n<\/tr>\n \n BSI<\/b><\/a><\/td>\n 2019<\/td>\n 34<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":315631,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[1177,2641],"product_tag":[],"class_list":{"0":"post-315624","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-83-080-01","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/315624","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/315631"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=315624"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=315624"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=315624"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}