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BSI PD ISO/TS 4985:2023

BSI PD ISO/TS 4985:2023

$142.49

Milk and milk products. Determination of alkaline phosphatase activity. Fluorimetric microplate method

Published By Publication Date Number of Pages
BSI 2023 30
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PDF Catalog

PDF Pages PDF Title
2 undefined
7 Forewords
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
10 5 Reagents
11 6 Apparatus and materials
12 7 Sampling
8 Preparation
8.1 Preparation of alkaline phosphatase-free sample
8.1.1 General
8.1.2 Alkaline phosphatase-free milk
8.1.3 Alkaline phosphatase-free cheese
13 8.2 Preparation of test sample
8.2.1 Milk samples
8.2.2 Cheese samples
9 Procedure
9.1 Instrument
9.1.1 Instrument settings
14 9.1.2 Test the linearity range of the instrument
9.1.3 Quality control
9.2 Calibration
9.2.1 General
9.2.2 Preparation of the working standard solutions
15 9.2.3 Preparation of the calibration curve
9.3 Determination
9.3.1 Milk samples
16 9.3.2 Cheese samples
17 9.3.3 Plate layout and fluorescence measurement
9.4 Test sample-related controls
9.4.1 Recommended negative and positive controls
18 9.4.2 Interfering substance test
9.4.3 Heat-stable microbial alkaline phosphatase control test
10 Calculation and expression of results
10.1 Calibration curve
19 10.2 Calculation of ALP activity of unknown samples
10.2.1 Milk samples
20 10.2.2 Cheese samples
21 10.2.3 Calculation example
22 10.3 Expression of results
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
11.3 Reproducibility
23 12 Test report
24 Annex A (informative) Precision data
25 Annex B (informative) Method comparison
27 Annex C (informative) Examples of preparation of test sample
29 Bibliography

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BSI PD ISO/TS 4985:2023
$142.49