Shopping Cart

No products in the cart.

BSI 23/30468623 DC 2023

$13.70

BS EN ISO 17174. Molecular biomarker analysis. DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments

Published By Publication Date Number of Pages
BSI 2023 27
Guaranteed Safe Checkout
Categories: ,

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

PDF Catalog

PDF Pages PDF Title
1 30468623-NC.pdf
3 ISO_DIS17174ed1-id85543EnquiryPDF(en).pdf
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
5 Reagents and materials
5.1 General
5.2 PCR reagents
13 6 Apparatus
7 Procedure
7.1 Sample preparation
14 7.2 DNA extraction
7.3 PCR
7.3.1 General
7.3.2 PCR setup
15 7.3.3 Temperature-time program
16 7.3.4 PCR controls
8 Evaluation
8.1 Evaluation of PCR products
8.2 Evaluation of the PCR results
17 8.3 Sequencing of PCR products
8.4 Evaluation of sequence data
8.5 Comparison of the sequence with public databases
8.5.1 General
18 8.5.2 Sequence comparison of cytb and/or cox1 DNA sequences with GenBank
8.5.3 Sequence comparison of cox1 DNA sequences with BOLD
19 9 Interpretation of database query results
20 10 Validation status and performance criteria
10.1 Collaborative study for the identification of fish species based on cytb sequence analysis
21 10.2 Collaborative study for the identification of fish species based on cox1 sequence analysis
22 11 Test report
23 Annex€A (informative) Practical laboratory experiences with the amplifiability of cytb and cox1 segments from tested fish species
26 Bibliography
BSI 23/30468623 DC 2023
$13.70